Interstate 4 which bisects Longwood provides easy access for Longwood businesses and residents to reach Orlando, Daytona Beach, and Tampa.
A 40-minute drive east on U.S. 4 intersects with U.S. 95, which travels along Florida's Atlantic coast and the eastern seaboard of the U.S. 90-minute drive west on U.S. 4 and U.S. 75 provides a direct route to Atlanta and Florida's Gulf Coast
Other major state roadways include SR. 434, 17/92, and 427.
Toll roads include State Road 528, State Road 408, State Road 417, and State Road 429 - 100 miles encircling Orlando. The Central Florida Expressway Authority manages this network of roads including large portions of a beltway around Orlando. The Authority operates an electronic toll collection system known as E-PASS, one of the first and most widely-used systems of its kind in the U.S.
The 12.5-mile Osceola Parkway links the international airport to major attractions and a half dozen regional arterial highways.
LYNX, an extensive public transportation system, serves Orange, Seminole, and Osceola counties. LYNX is the first transit agency in the nation to build, produce and utilize its own B-20 Biodiesel blend for bus fleet conversion and the first to distribute B-20 to other regional agencies.