- Interstate 4 which bisects Longwood provides easy access for Longwood businesses and residents to reach Orlando, Daytona Beach, and Tampa.
- A 40 minute drive east on U.S. 4 and it intersects with U.S. 95, which travels along Florida's Atlantic coast and the eastern seaboard of the U.S. A 90 minute drive west on U.S. 4 and U.S. 75 provides a direct route to Atlanta and Florida's Gulf Coast
- Other major state roadways include SR. 434, 17/92 and 427.
- Toll roads include State Road 528, State Road 408, State Road 417 and State Road 429 - 100 miles encircling Orlando. The Orlando-Orange County Expressway Authority (OOCEA) manages this network of roads including large portions of a beltway around Orlando. The OOCEA operates an electronic toll collection system known as E-PASS, one of the first and most widely-used systems of its kind in the U.S.
- The 12.5-mile Osceola Parkway links the international airport to major attractions and a half dozen regional arterial highways.
- LYNX, an extensive public transportation system, serves Orange, Seminole and Osceola counties. LYNX is the first transit agency in the nation to build, produce and utilize its own B-20 Biodiesel blend for bus fleet conversion and the first to distribute B-20 to other regional agencies.
- Access the 2014 Florida Transportation Map.